Human RAGE ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

RAGE

Applications

Sandwhich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sample type

Serum, plasma, urine, cell culture supernatants, and tissue homogenates.

Detection type

Colorimetric

Platform

Microplate

Product range

130-4000 pg/ml

Principiul testului

This RAGE enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-RAGE Antibody. Standards or samples are then added to the microtire plate wells and RAGE, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of RAGE present in the sample, Anti-RAGE Antibody (HRP) is added to each well to "sandwich" the RAGE immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain RAGE will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Storage

Store at 2-8°C for 6 months.