Imagine indisponibilăHuman Peroxiredoxin 1/PAG ELISA Kit
Pe scurt
Human Peroxiredoxin 1/PAG ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea Peroxiredoxin 1/PAG, cu reactivitate declarată pentru Human. Măsoară în domeniul 15.625-1000 pg/ml, are o sensibilitate de 9.375 pg/ml, folosește detecție Colorimetric Detection (OD450) și se poate folosi pe probe de tip Serum, plasma, tissue homogenates, and other biological fluids. Se livrează în mărimea 96T, la prețul de 4.392,30 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Human Peroxiredoxin 1/PAG ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Peroxiredoxin 1/PAG in serum, plasma, tissue homogenates, and other biological fluids.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- Peroxiredoxin 1/PAG
- Applications
- Sandwich ELISA
- Reactivity
- Human
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 9.375 pg/ml
- Cross reactivity
- Specific for Peroxiredoxin 1/PAG with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 15.625-1000 pg/ml
- Assay Time
- 4 hours
- Principiul testului
- Human Peroxiredoxin 1/PAG ELISA Kit (A77180) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Peroxiredoxin 1/PAG in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for Peroxiredoxin 1/PAG. Standards and samples are added to the wells, where Peroxiredoxin 1/PAG binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and an HRP-conjugated detection antibody specific for Peroxiredoxin 1/PAG is added to the wells. The detection antibody binds the captured Peroxiredoxin 1/PAG to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Peroxiredoxin 1/PAG captured in each well. The concentration of Peroxiredoxin 1/PAG is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
Documentație
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