Human PCNA ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

48T · 96T

Țintă

PCNA

Applications

Sandwich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sensitivity

0.123 ng/ml

Sample type

Tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Detection type

Colourmetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

0.312-20 ng/ml

Assay Time

4h 30m

Principiul testului

Human PCNA ELISA Kit (A1921) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human PCNA in tissue homogenates, cell lysates, cell culture supernates or other biological fluids. An antibody specific for PCNA has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the PCNA present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-PCNA Antibody, which binds the captured PCNA present in each well. HRP-Avidin conjugate is then added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of PCNA captured in each well. The concentration of PCNA can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Storage

Store kit components at +4°C or -20°C as instructed. Do not use past expiration date!

Documentație

• Manual de utilizare (PDF)se deschide pe site-ul producătorului