Imagine indisponibilăHuman Osteoprotegerin ELISA Kit
Pe scurt
Human Osteoprotegerin ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea Osteoprotegerin, cu reactivitate declarată pentru Human. Măsoară în domeniul 62.5-4000 pg/ml, are o sensibilitate de 37.5 pg/ml, folosește detecție Colorimetric Detection (OD450) și se poate folosi pe probe de tip Plasma, cell culture supernatant, cell or tissue lysate, other liquid samples. Se livrează în mărimea 96T, la prețul de 3.746,77 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Human Osteoprotegerin ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Osteoprotegerin in plasma, cell culture supernatant, cell or tissue lysate and other liquid samples.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- Osteoprotegerin
- Applications
- Sandwich ELISA
- Reactivity
- Human
- Recommended dilutions
- Validated sample dilutions include Healthy serum (undiluted) and Healthy human saliva (undiluted).
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 37.5 pg/ml
- Cross reactivity
- Specific for Osteoprotegerin with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 62.5-4000 pg/ml
- Assay Time
- 2 hours
- Principiul testului
- Human Osteoprotegerin ELISA Kit (A335576) is a 2 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Osteoprotegerin in plasma, cell culture supernatant, cell or tissue lysate, other liquid samples. This QuickRun format uses a pre-coated 96-well microtiter plate and optimized ready-to-use reagents to reduce assay time. Standards and samples are added to the wells, where Osteoprotegerin binds to the immobilized capture antibody. Assay-specific biotinylated detection antibody and HRP-Streptavidin reagents are then added according to the optimized QuickRun workflow. These reagents form an antibody-antigen-antibody sandwich complex with the captured Osteoprotegerin. TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Osteoprotegerin captured in each well. The concentration of Osteoprotegerin is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
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