Imagine indisponibilăHuman NT-ProCNP ELISA Kit
Pe scurt
Human NT-ProCNP ELISA Kit este un kit ELISA de tip Competitive (quantitative) pentru măsurarea NT-ProCNP, cu reactivitate declarată pentru Human. Măsoară în domeniul 15.6-1000 pg/ml, are o sensibilitate de 4.42 pg/ml, folosește detecție Colourmetric și se poate folosi pe probe de tip Serum, plasma, tissue homogenates or other biological fluids. Se livrează în mărimile 48T și 96T, la prețuri între 4.259,20 și 5.523,65 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Human NT-ProCNP ELISA Kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of human NT-ProCNP in serum, plasma, tissue homogenates or other biological fluids.
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Specificații
- Brand
- Antibodies
- Mărimi disponibile
- 48T · 96T
- Țintă
- NT-ProCNP
- Applications
- Competitive ELISA
- Reactivity
- Human
- Assay type
- Competitive (quantitative)
- Sensitivity
- 4.42 pg/ml
- Detection type
- Colourmetric
- Product range
- 15.6-1000 pg/ml
- Assay Time
- 2h 30m
- Principiul testului
- Human NT-ProCNP ELISA Kit (A7941) employs the competitive enzyme immunoassay technique for the quantitative measurement of human NT-ProCNP in serum, plasma, tissue homogenates or other biological fluids. The 96-well microtiter plate has been pre-coated with NT-ProCNP antigen. During the incubation, NT-ProCNP present in the samples or standards competes with the fixed amount of immobilized NT-ProCNP for binding sites on the Biotinylated Anti-NT-ProCNP Antibody. The more NT-ProCNP present in a sample or standard, the less Biotinylated Anti-NT-ProCNP Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-NT-ProCNP Antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of NT-ProCNP present in each sample or standard. The concentration of NT-ProCNP can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
Documentație
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