Imagine indisponibilăHuman NT-proBNP ELISA Kit
Pe scurt
Human NT-proBNP ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea NT proBNP, cu reactivitate declarată pentru Human. Măsoară în domeniul 39.063-2500 pg/ml, are o sensibilitate de 23.438 pg/ml și folosește detecție Colorimetric Detection (OD450). Se livrează în mărimea 96T, la prețul de 3.746,77 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Human NT-proBNP ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human NT-proBNP in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.
Citește mai multArată mai puțin
Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- NT proBNP
- Applications
- Sandwich ELISA
- Reactivity
- Human
- Recommended dilutions
- Validated sample dilutions include Healthy serum (undiluted) and serum from heart failure patients(n=4) (1:2-1:10).
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 23.438 pg/ml
- Sample type
- Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.
- Cross reactivity
- Specific for NT-proBNP with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 39.063-2500 pg/ml
- Assay Time
- 2 hours
- Principiul testului
- Human NT-proBNP ELISA Kit (A335611) is a 2 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human NT-proBNP in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. This QuickRun format uses a pre-coated 96-well microtiter plate and optimized ready-to-use reagents to reduce assay time. Standards and samples are added to the wells, where NT-proBNP binds to the immobilized capture antibody. Assay-specific biotinylated detection antibody and HRP-Streptavidin reagents are then added according to the optimized QuickRun workflow. These reagents form an antibody-antigen-antibody sandwich complex with the captured NT-proBNP. TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of NT-proBNP captured in each well. The concentration of NT-proBNP is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
Produse similare
- Imagine indisponibilă
- Imagine indisponibilă
- Imagine indisponibilă
- Imagine indisponibilă
- Imagine indisponibilăAntibodiesA331122
Recombinant Human NT-proBNP Protein (C-terminal Human Fc Tag)
1.996,50 – 2.861,65 lei1.650,00 – 2.365,00 leiTVA inclus (21%)fără TVA
- Imagine indisponibilă
- Imagine indisponibilă
- Imagine indisponibilă
- Imagine indisponibilă
- Imagine indisponibilă
- Imagine indisponibilă
- Imagine indisponibilă