Human Muscarinic Acetylcholine Receptor ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Muscarinic Acetylcholine Receptor

Applications

Sandwich ELISA

Reactivity

Human

Recommended dilutions

Validated sample dilutions include SH-SY5Y cell lysate (1:5-1:10) and U-118MG cell lysate (1:2).

Assay type

Sandwich (quantitative)

Sensitivity

0.375 ng/ml

Sample type

Serum, plasma, tissue homogenates, and other biological fluids.

Cross reactivity

Specific for Muscarinic Acetylcholine Receptor with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

0.625-40 ng/ml

Assay Time

4 hours

Principiul testului

Human Muscarinic Acetylcholine Receptor ELISA Kit (A74884) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Muscarinic Acetylcholine Receptor in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for Muscarinic Acetylcholine Receptor. Standards and samples are added to the wells, where Muscarinic Acetylcholine Receptor binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for Muscarinic Acetylcholine Receptor is added to the wells. The detection antibody binds the captured Muscarinic Acetylcholine Receptor to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Muscarinic Acetylcholine Receptor captured in each well. The concentration of Muscarinic Acetylcholine Receptor is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.

Documentație

• Manual de utilizare (PDF)se deschide pe site-ul producătorului