Human Macrophage Colony Stimulating Factor ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

96T · 192T · 192T x 3

Țintă

Macrophage Colony Stimulating Factor

Applications

Sandwich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 15 pg/ml

Sample type

Serum, plasma and cell culture supernatant.

Cross reactivity

This assay has shown no cross-reactivity with other cytokines tested.

Detection type

Colorimetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

120-4000 pg/ml

Assay Time

4h

Specificitate

This sandwich ELISA can detect both natural and recombinant human M-CSF.

Principiul testului

This M-CSF enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to M-CSF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for M-CSF and incubated. If present, M-CSF will bind and become immobilized by the antibody pre-coated on the wells and then become “sandwiched” by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound MCSF and other components of the sample. In order to quantitatively determine the amount of M-CSF present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits, each having a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain M-CSF, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. In order to measure the concentration of M-CSF in the samples, this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus M-CSF concentration (pg/mL). The concentration of M-CSF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Storage

Shipped and stored at 4°C.