Human Leptin Receptor ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Leptin receptor

Applications

Sandwich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sensitivity

3.26 pg/ml

Sample type

Cell culture supernatant, serum and plasma.

Cross reactivity

This ELISA kit has shown no significant cross-reactivity.

Detection type

Colorimetric

Platform

Microplate

Product range

62.50 - 4000 pg/ml

Recovery

98% (Average Recovery from Serum Samples).

Specificitate

This kit can assay both native and recombinant human Leptin Receptor.

Principiul testului

Human Leptin Receptor ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Leptin R in the samples. A monoclonal antibody specific for human Leptin R has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for Leptin R, and Leptin R present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Leptin R bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Storage

Store at +4°C. Do not use past expiration date!