Human Leptin ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Leptin

Applications

Sandwich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sample type

Plasma, serum, and lysates.

Cross reactivity

No reported cross-reactivity.

Detection type

Colorimetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

15-1000 pg/ml

Principiul testului

This Human Leptin ELISA Kit allows for the quantitative determination of natural or recombinant Leptin within any experimental sample including cell lysates, serum, and plasma. This Human Leptin ELISA Kit applies a technique called quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for Leptin. Standards or samples are then added to the appropriate microtiter plate wells and incubated. If present, Leptin will bind and become immobilized by the antibody pre-coated on the wells. Leptin then becomes “sandwiched” by the primary capture antibodies and the secondary detection antibodies subsequently added. After incubation and “sandwiching” of Leptin, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.

Storage

Store at + 4°C. Stable for 3-6 months.