Human IL1 beta ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

IL1 beta

Applications

Sandwich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sensitivity

0.15 pg/ml

Sample type

Cell culture supernatant, serum and plasma.

Cross reactivity

This ELISA kit has shown no significant cross-reactivity.

Detection type

Colorimetric

Platform

Microplate

Product range

7.81 - 500 pg/ml

Recovery

105% (Average Recovery from Serum Samples).

Specificitate

This kit can assay both native and recombinant human IL-1ß.

Principiul testului

Human IL-1ß ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human IL-1ß in the samples. A monoclonal antibody specific for human IL-1ß has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for IL-1ß, and IL-1ß present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-1ß bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Storage

Store at +4°C. Do not use past expiration date!