Human IL-8 ELISA Kit (High Sensitivity)

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

IL-8

Applications

Sandwich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sensitivity

0.29 pg/ml

Sample type

Serum, plasma and other biological fluids.

Cross reactivity

Specific for IL-8 with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

0.47-30 pg/ml

Assay Time

3h 30m

Principiul testului

Human IL-8 ELISA Kit (A323171) employs the sandwich Enzyme-Linked Immunoassay technique for the quantitative measurement of low-abundance human IL-8 in serum, plasma, and other biological fluids. An antibody specific for IL-8 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the IL-8 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-IL-8 Antibody, which binds the captured IL-8 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of IL-8 captured in each well. The concentration of IL-8 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.