Imagine indisponibilăHuman IL-6 ELISA Kit
Pe scurt
Human IL-6 ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea IL-6, cu reactivitate declarată pentru Human. Măsoară în domeniul 6.25-200 pg/ml, are o sensibilitate de < 2 pg/ml, folosește detecție Colorimetric și se poate folosi pe probe de tip Serum, plasma, and cell culture supernatants. Se livrează în mărimea 96T, la prețul de 6.155,88 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Human IL-6 ELISA kit includes pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB substrate, stop reagent, and a detailed assay procedure. The kit is designed for sensitive and quantitative detection of Human IL-6 in biological samples.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- IL-6
- Applications
- Sandwhich ELISA
- Reactivity
- Human
- Conjugate
- Unconjugated
- Assay type
- Sandwich (quantitative)
- Sensitivity
- < 2 pg/ml
- Cross reactivity
- This product shows no cross reactivity with other human cytokines. No interference with soluble receptor gp80.
- Detection type
- Colorimetric
- Platform
- Pre-coated Microplate (1 x 96)
- Product range
- 6.25-200 pg/ml
- Assay Time
- 1h 45m
- Specificitate
- This product recognizes both natural and recombinant human IL-6.
- Principiul testului
- Human IL-6 ELISA Kit (A341797) is a sandwich ELISA designed for the sensitive and quantitative measurement of human IL-6 in serum, plasma and cell culture supernatants. The assay utilizes a 96-well microtiter plate pre-coated with an antibody specific for IL-6. Standards and samples are added to the wells, allowing IL-6 present in the samples to bind to the immobilized capture antibody. After washing, a biotinylated detection antibody specific for IL-6 is added, forming an antibody–antigen–antibody complex. Unbound detection antibody is removed by washing, followed by the addition of streptavidin conjugated to horseradish peroxidase (HRP). Following incubation and washing, TMB substrate is added, resulting in a colorimetric reaction that produces a blue color which turns yellow upon addition of stop solution. The intensity of the color is directly proportional to the concentration of IL-6 in the sample. The concentration of IL-6 is determined by measuring the absorbance at 450 nm and comparing the results to the standard curve.
- Storage
- Store at 4°C.
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