Human IL-5 ELISpot Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

IL-5

Applications

ELISpot

Reactivity

Human

Conjugate

Unconjugated

Sample type

Peripheral blood mononuclear cells (PBMCs).

Cross reactivity

This product shows no cross reactivity with other human cytokines.

Detection type

Colorimetric

Platform

Pre-coated Microplate (1 x 96)

Assay Time

3h after cell stimulation

Specificitate

This product recognizes both natural and recombinant human IL-5.

Principiul testului

Human IL-5 ELISpot Kit (A341815) is a sandwich ELISA designed for the sensitive and quantitative measurement of human IL-5 in peripheral blood mononuclear cells (pbmcs). The assay utilizes a 96-well microtiter plate pre-coated with an antibody specific for IL-5. Standards and samples are added to the wells, allowing IL-5 present in the samples to bind to the immobilized capture antibody. After washing, a biotinylated detection antibody specific for IL-5 is added, forming an antibody–antigen–antibody complex. Unbound detection antibody is removed by washing, followed by the addition of streptavidin conjugated to horseradish peroxidase (HRP). Following incubation and washing, TMB substrate is added, resulting in a colorimetric reaction that produces a blue color which turns yellow upon addition of stop solution. The intensity of the color is directly proportional to the concentration of IL-5 in the sample. The concentration of IL-5 is determined by measuring the absorbance at 450 nm and comparing the results to the standard curve.

Storage

Store at 4°C.