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Human IL-28B ELISA Kit
Pe scurt
Human IL-28B ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea IL-28B, cu reactivitate declarată pentru Human. Măsoară în domeniul 15.625-1000 pg/ml, are o sensibilitate de 9.375 pg/ml și folosește detecție Colorimetric Detection (OD450). Se livrează în mărimea 96T, la prețul de 3.746,77 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Human IL-28B ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human IL-28B in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- IL-28B
- Applications
- Sandwich ELISA
- Reactivity
- Human
- Recommended dilutions
- Validated sample dilutions include Healthy serum (undiluted) and Human PBL culture supernatants (10µg/mL PHA and 10 ng/mL recombinant human IFN-?, stimulation for 24 hours) (undiluted).
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 9.375 pg/ml
- Sample type
- Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.
- Cross reactivity
- Specific for IL-28B with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 15.625-1000 pg/ml
- Assay Time
- 2 hours
- Principiul testului
- Human IL-28B ELISA Kit (A336028) is a 2 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human IL-28B in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. This QuickRun format uses a pre-coated 96-well microtiter plate and optimized ready-to-use reagents to reduce assay time. Standards and samples are added to the wells, where IL-28B binds to the immobilized capture antibody. Assay-specific biotinylated detection antibody and HRP-Streptavidin reagents are then added according to the optimized QuickRun workflow. These reagents form an antibody-antigen-antibody sandwich complex with the captured IL-28B. TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of IL-28B captured in each well. The concentration of IL-28B is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
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