Imagine indisponibilăHuman IL-17F ELISpot Kit
Pe scurt
Human IL-17F ELISpot Kit este un kit ELISA pentru măsurarea IL-17F, cu reactivitate declarată pentru Human. Folosește detecție Colorimetric și se poate folosi pe probe de tip Peripheral blood mononuclear cells (PBMCs). Se livrează în mărimea 96T, la prețul de 4.591,95 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Human IL-17F ELISpot kit includes pre-coated PVDF plates, detection antibody, alkaline phosphatase conjugate, BSA, and BCIP/NBT ready-to-use substrate buffer. The kit is designed for sensitive, single-cell ELISpot detection of Human IL-17F secretion in PBMCs.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- IL-17F
- Applications
- ELISpot
- Reactivity
- Human
- Conjugate
- Unconjugated
- Sample type
- Peripheral blood mononuclear cells (PBMCs).
- Detection type
- Colorimetric
- Platform
- Pre-coated Microplate (1 x 96)
- Assay Time
- 3h after cell stimulation
- Specificitate
- This product recognizes natural human IL-17F.
- Principiul testului
- Human IL-17F ELISpot Kit (A341820) is a sandwich ELISA designed for the sensitive and quantitative measurement of human IL-17F in peripheral blood mononuclear cells (pbmcs). The assay utilizes a 96-well microtiter plate pre-coated with an antibody specific for IL-17F. Standards and samples are added to the wells, allowing IL-17F present in the samples to bind to the immobilized capture antibody. After washing, a biotinylated detection antibody specific for IL-17F is added, forming an antibody–antigen–antibody complex. Unbound detection antibody is removed by washing, followed by the addition of streptavidin conjugated to horseradish peroxidase (HRP). Following incubation and washing, TMB substrate is added, resulting in a colorimetric reaction that produces a blue color which turns yellow upon addition of stop solution. The intensity of the color is directly proportional to the concentration of IL-17F in the sample. The concentration of IL-17F is determined by measuring the absorbance at 450 nm and comparing the results to the standard curve.
- Storage
- Store at 4°C.
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