Imagine indisponibilăHuman IL-17A ELISA Kit
Pe scurt
Human IL-17A ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea IL-17A, cu reactivitate declarată pentru Human. Măsoară în domeniul 31.25 - 2000 pg/ml, are o sensibilitate de 18.75 pg/ml și folosește detecție Colorimetric Detection (OD450). Se livrează în mărimea 96T, la prețul de 3.746,77 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Human IL-17A ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human IL-17A in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- IL-17A
- Applications
- Sandwich ELISA
- Reactivity
- Human
- Recommended dilutions
- Validated sample dilutions include Healthy serum (undiluted or 1:2) and Human PMBC culture supernatants (10µg/mL PHA stimulation for 24 hours) (undiluted).
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 18.75 pg/ml
- Sample type
- Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.
- Cross reactivity
- Specific for IL-17A with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 31.25 - 2000 pg/ml
- Assay Time
- 2 hours
- Principiul testului
- Human IL-17A ELISA Kit (A336168) is a 2 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human IL-17A in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. This QuickRun format uses a pre-coated 96-well microtiter plate and optimized ready-to-use reagents to reduce assay time. Standards and samples are added to the wells, where IL-17A binds to the immobilized capture antibody. Assay-specific biotinylated detection antibody and HRP-Streptavidin reagents are then added according to the optimized QuickRun workflow. These reagents form an antibody-antigen-antibody sandwich complex with the captured IL-17A. TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of IL-17A captured in each well. The concentration of IL-17A is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
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