Imagine indisponibilăHuman IL-12RB1 ELISA Kit
Pe scurt
Human IL-12RB1 ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea IL-12RB1, cu reactivitate declarată pentru Human. Măsoară în domeniul 0.156-10 ng/ml, are o sensibilitate de 0.071 ng/ml, folosește detecție Colourmetric și se poate folosi pe probe de tip Serum, plasma, tissue homogenates or other biological fluids. Se livrează în mărimile 48T și 96T, la prețuri între 3.726,80 și 4.758,33 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Human IL-12RB1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human IL-12RB1 in serum, plasma, tissue homogenates or other biological fluids.
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Specificații
- Brand
- Antibodies
- Mărimi disponibile
- 48T · 96T
- Țintă
- IL-12RB1
- Applications
- Sandwich ELISA
- Reactivity
- Human
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 0.071 ng/ml
- Detection type
- Colourmetric
- Product range
- 0.156-10 ng/ml
- Assay Time
- 4h 30m
- Principiul testului
- Human IL-12RB1 ELISA Kit (A7671) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human IL-12RB1 in serum, plasma, tissue homogenates or other biological fluids. An antibody specific for IL-12RB1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the IL-12RB1 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-IL-12RB1 Antibody, which binds the captured IL-12RB1 present in each well. HRP-Avidin conjugate is then added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of IL-12RB1 captured in each well. The concentration of IL-12RB1 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
Documentație
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