Human IL-10 ELISpot Kit

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

IL-10

Applications

ELISpot

Reactivity

Human

Host

Human

Assay type

sandwich ELISA

Sample type

Peripheral blood mononuclear cells (PBMCs) in single-cell suspension, capable of cytokine secretion.

Detection type

Colorimetric

Platform

Precoated PVDF Microplate

Assay Time

Assay time: 3 hours. Sample incubation time: 16-24 hours.

Specificitate

This ELISpot Kit specifically targets Human IL-10.

Principiul testului

The Human IL-10 ELISpot Kit (A334657) employs the double-antibody sandwich ELISpot technique for the detection of IL-10-secreting cells. A capture antibody specific for IL-10 has been pre-coated onto a 96-well microplate. Test cells, such as PBMCs, are added into the wells, and upon stimulation with a cell stimulant, IL-10 secreted by individual cells is captured by the immobilized antibody. A biotinylated anti-IL-10 detection antibody, which also binds the captured cytokine, and Streptavidin-HRP, which binds the biotinylated detection antibody, are then added, and the plate is incubated. Following incubation, unbound detection antibody and Streptavidin-HRP are removed by washing, and AEC substrate solution is added. Colored spots develop at the sites of cytokine secretion in proportion to the number of IL-10-producing cells. The intensity and number of spots can then be quantified using an ELISpot reader. This ready-to-use kit includes pre-coated plates, detection antibody, Streptavidin-HRP, AEC coloring system, dilution buffer, cell stimulant, and washing buffer. For research use only. Not for diagnostic purposes.

Storage

Shipped at 4°C. The kit should be stored at 2-8°C. Shelf life is 12 months from the date of receipt.

Documentație

• Manual de utilizare (PDF)se deschide pe site-ul producătorului