Human IL-1 alpha ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

IL-1 alpha

Applications

Sandwich ELISA

Reactivity

Human

Recommended dilutions

Validated sample dilutions include Healthy serum (undiluted) and Human peripheral blood Cell Culture Supernatants (10µg/mL PHA stimulation for 24 hours) (1:2-1:5).

Assay type

Sandwich (quantitative)

Sensitivity

2.344 pg/ml

Sample type

Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.

Cross reactivity

Specific for IL-1 alpha with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

3.906-250 pg/ml

Assay Time

2 hours

Principiul testului

Human IL-1 alpha ELISA Kit (A335548) is a 2 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human IL-1 alpha in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. This QuickRun format uses a pre-coated 96-well microtiter plate and optimized ready-to-use reagents to reduce assay time. Standards and samples are added to the wells, where IL-1 alpha binds to the immobilized capture antibody. Assay-specific biotinylated detection antibody and HRP-Streptavidin reagents are then added according to the optimized QuickRun workflow. These reagents form an antibody-antigen-antibody sandwich complex with the captured IL-1 alpha. TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of IL-1 alpha captured in each well. The concentration of IL-1 alpha is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.