Human IgE ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

IgE

Applications

ELISA

Reactivity

Human

Formulation

Antibodies are supplied in Phosphate Buffered Saline with Tris-HCl.

Host

Rabbit

Product form

Recombinant protein is supplied lyophilized; antibodies and buffer are supplied in liquid form.

Assay type

Sandwich (quantitative)

Sensitivity

0.050 ng/ml

Sample type

Serum, plasma and cell culture supernatant.

Detection type

Colorimetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

0.156 ng/ml - 10 ng/ml

Assay Time

1 hour

Recovery

Cell culture supernatant: 91%; Serum: 102%; Plasma: 117%

Imunogen

The immunogens for the antibodies in this kit are recombinant IgA.

Principiul testului

Human IgE ELISA Kit (A334815) employs a one-step sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of human IgE in serum, plasma, and cell culture supernatant. A 96-well microtiter plate is provided pre-coated with an antibody specific for IgE. Standards and test samples are added to the wells, allowing IgE to bind to the immobilized capture antibody. A prepared antibody mixture is added and incubated to form antibody–antigen complexes. After incubation, unbound material is removed by washing, and TMB substrate is added to develop colour through an HRP-catalysed reaction. The reaction is stopped with acidic stop solution, producing a yellow endpoint signal. The intensity of the colour is proportional to the amount of IgE captured in each well. The concentration of IgE is determined by measuring absorbance at 450 nm and interpolating from the standard curve.

Storage

Shipped at +4 °C. For short-term storage, keep at +4 °C. Shelf life is 12 months from the date of receipt or reconstitution.