Human IFN gamma ELISpot Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

IFN gamma

Applications

ELISpot

Reactivity

Human

Conjugate

Unconjugated

Sample type

Peripheral blood mononuclear cells (PBMCs).

Cross reactivity

This product shows no cross reactivity with other human cytokines. Cross reactivity with simian IFN gamma.

Detection type

Colorimetric

Platform

Pre-coated Microplate (1 x 96)

Assay Time

3h after cell stimulation

Specificitate

This product recognizes natural human IFN-g.

Principiul testului

Human IFN gamma ELISpot Kit (A341811) is a sandwich ELISA designed for the sensitive and quantitative measurement of human IFN gamma in peripheral blood mononuclear cells (pbmcs). The assay utilizes a 96-well microtiter plate pre-coated with an antibody specific for IFN gamma. Standards and samples are added to the wells, allowing IFN gamma present in the samples to bind to the immobilized capture antibody. After washing, a biotinylated detection antibody specific for IFN gamma is added, forming an antibody–antigen–antibody complex. Unbound detection antibody is removed by washing, followed by the addition of streptavidin conjugated to horseradish peroxidase (HRP). Following incubation and washing, TMB substrate is added, resulting in a colorimetric reaction that produces a blue color which turns yellow upon addition of stop solution. The intensity of the color is directly proportional to the concentration of IFN gamma in the sample. The concentration of IFN gamma is determined by measuring the absorbance at 450 nm and comparing the results to the standard curve.

Storage

Store at 4°C.