Human IFN-? ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

IFN gamma

Applications

Sandwich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sensitivity

0.30 pg/ml

Sample type

Cell culture supernatant, serum and plasma.

Cross reactivity

This ELISA kit has shown no significant cross-reactivity.

Detection type

Colorimetric

Platform

Microplate

Product range

7.81 - 500 pg/ml

Recovery

92% (Average Recovery from Serum Samples).

Specificitate

This kit can assay both native and recombinant human IFN-?.

Principiul testului

Human IFN-? ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human IFN-? in the samples. A monoclonal antibody specific for human IFN-? has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for IFN-?, and IFN-? present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IFN-? bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Storage

Store at +4°C. Do not use past expiration date!