Human HPV16 L1 ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

HPV16 L1

Applications

Indirect ELISA

Reactivity

Human

Assay type

Indirect (qualitative)

Sample type

Serum, plasma, and cell culture supernatants.

Detection type

Colorimetric

Platform

Microplate

Principiul testului

This ELISA kit is indirect enzyme-linked immune-sorbent assay technology. The recombinant HPV16 L1 antigens were pre-coated onto 96-well plates. The test samples were added to the wells and incubated subsequently, the HPV16 L1 antibodies in the test samples combined to the pre-coated antigens specifically, then followed by washing with wash buffer to remove unspecific components. Next, the HRP conjugated antibodies were added and incubated to bind to human antibodies in plate, the unbound conjugates were washed away with wash buffer. TMB substrate were used to visualize HRP enzymatic reaction. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Storage

Store at 2-8°C for 6 months or at -20°C for 12 months Store secondary antibody at 2-8°C.