Human HIF1 beta ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

HIF1 beta

Applications

Sandwich ELISA

Reactivity

Human

Recommended dilutions

Serum and plasma samples may affect test results and should be diluted at least 1:2 with Sample Dilution Buffer prior to testing. Validated sample dilutions include Healthy serum (1:2) and MCF-7 cell lysate (stimulated with 375µM CoCl2 for 24h) (1:2).

Assay type

Sandwich (quantitative)

Sensitivity

0.094 ng/ml

Sample type

Serum, plasma, tissue homogenates, and other biological fluids.

Cross reactivity

Specific for HIF1 beta with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

0.156-10 ng/ml

Assay Time

4 hours

Principiul testului

Human HIF1 beta ELISA Kit (A77710) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human HIF1 beta in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for HIF1 beta. Standards and samples are added to the wells, where HIF1 beta binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for HIF1 beta is added to the wells. The detection antibody binds the captured HIF1 beta to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of HIF1 beta captured in each well. The concentration of HIF1 beta is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.

Documentație

• Manual de utilizare (PDF)se deschide pe site-ul producătorului