Human Hepatitis D Virus IgM ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Hepatitis D Virus IgM

Applications

Trapping ELISA

Reactivity

Human

Assay type

Trapping (qualitative)

Sample type

Serum, plasma, tissue homogenates, and other biological fluids.

Cross reactivity

Specific for Hepatitis D Virus IgM with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

Qualitative

Assay Time

3 hours

Principiul testului

Human Hepatitis D Virus IgM ELISA Kit (A75107) is a 3 hour trapping Enzyme-Linked Immunosorbent Assay (tELISA) designed for the in vitro qualitative detection of human Hepatitis D Virus IgM in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an assay-specific trapping reagent. Samples are added to the wells, where Hepatitis D Virus IgM is captured by the immobilized trapping reagent. Following incubation, unbound components are removed by washing and an assay-specific detection reagent is added. This detection reagent binds the captured Hepatitis D Virus IgM to form a trapped immune complex. After further incubation and washing, TMB substrate solution is added to visualize the enzyme-mediated reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The presence or absence of colour indicates the presence or absence of Hepatitis D Virus IgM in the sample.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.