Human HEK293 Host Cell Protein ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

HEK293 Host Cell Protein

Applications

Sandwich ELISA

Reactivity

Human

Recommended dilutions

Validated sample dilutions include HEK293 Cell Culture Supernatant (48h) (1:2) and HEK293 cell lysate (100µg/mL BCA) (1:40).

Assay type

Sandwich (quantitative)

Sensitivity

46.875 ng/ml

Sample type

Cell Culture Supernatant, Cell or tissue lysate and other liquid samples.

Cross reactivity

Specific for HEK293 Host Cell Protein with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

78.125-5000 ng/ml

Assay Time

4 hours

Principiul testului

Human HEK293 Host Cell Protein ELISA Kit (High Range) (A302968) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human HEK293 Host Cell Protein in cell culture supernatant, cell or tissue lysate, and other liquid samples. A 96-well microtiter plate has been pre-coated with an antibody specific for HEK293 Host Cell Protein. Standards and samples are added to the wells, where HEK293 Host Cell Protein binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for HEK293 Host Cell Protein is added to the wells. The detection antibody binds the captured HEK293 Host Cell Protein to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of HEK293 Host Cell Protein captured in each well. The concentration of HEK293 Host Cell Protein is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.

Documentație

• Manual de utilizare (PDF)se deschide pe site-ul producătorului