Human Gliadin IgG4 ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Gliadin IgG4

Applications

Capture ELISA

Reactivity

Human

Assay type

Capture (quantitative)

Sensitivity

0.938 ng/ml

Sample type

Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.

Cross reactivity

Specific for Gliadin IgG4 with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

1.56 - 100 ng/ml

Assay Time

3 hours

Principiul testului

Human Gliadin IgG4 ELISA Kit (A334963) is a capture Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of human Gliadin IgG4 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. A 96-well microtiter plate has been pre-coated with a capture antibody specific for Gliadin IgG4. Standards and samples are added to the wells, where Gliadin IgG4 present in each sample binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and an assay-specific detection reagent is added to the wells. This detection reagent binds the captured Gliadin IgG4 to form a capture immune complex. After further incubation and washing, TMB substrate solution is added to visualize the enzyme-mediated reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Gliadin IgG4 captured in each well. The concentration of Gliadin IgG4 is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.