Human EpCAM ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

EPCAM

Applications

Sandwich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sensitivity

8.39 pg/ml

Sample type

Cell culture supernatant, serum and plasma.

Cross reactivity

This ELISA kit has shown no significant cross-reactivity.

Detection type

Colorimetric

Platform

Microplate

Product range

31.25 - 2000 pg/ml

Recovery

85% (Average Recovery from Serum Samples).

Specificitate

This kit can assay both native and recombinant human EpCAM.

Principiul testului

Human EpCAM ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human EpCAM in the samples. A monoclonal antibody specific for human EpCAM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells for the first incubation. After washing away any unbound substances, a HRP-linked detect antibody specific for EpCAM is added to the wells. After washing, substrate solution is added to the wells and color develops in proportion to the amount of EpCAM bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage

Store at +4°C. Do not use past expiration date!