Human EMAP II/AIMP1 ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

48T · 96T

Țintă

EMAP II/AIMP1

Applications

Sandwich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sensitivity

43 pg/ml

Sample type

Tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Detection type

Colourmetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

125-8000 pg/ml

Assay Time

4h 30m

Principiul testului

Human EMAP II/AIMP1 ELISA Kit (A5676) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human EMAP II/AIMP1 in tissue homogenates, cell lysates, cell culture supernates or other biological fluids. An antibody specific for EMAP II/AIMP1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the EMAP II/AIMP1 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-EMAP II/AIMP1 Antibody, which binds the captured EMAP II/AIMP1 present in each well. HRP-Avidin conjugate is then added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of EMAP II/AIMP1 captured in each well. The concentration of EMAP II/AIMP1 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Storage

Store kit components at +4°C or -20°C as instructed. Do not use past expiration date!

Documentație

• Manual de utilizare (PDF)se deschide pe site-ul producătorului