Human E Selectin ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

E Selectin

Applications

Sandwhich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 4 pg/ml

Sample type

Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.

Detection type

Colorimetric

Platform

Microplate

Product range

125-8000 pg/ml

Principiul testului

This E Selectin enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-E Selectin Antibody. Standards or samples are then added to the microtire plate wells and E Selectin, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of E Selectin present in the sample, Anti-E Selectin Antibody (Biotin) is added to each well to "sandwich" the E Selectin immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain E Selectin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Storage

Store at 2-8°C for 4 months or at -20°C for 8 months.