Human Diphosphoinositol Polyphosphate Phosphohydrolase 3 beta ELISA Kit

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

48T · 96T

Țintă

Diphosphoinositol Polyphosphate Phosphohydrolase 3 beta

Applications

Competitive ELISA

Reactivity

Human

Assay type

Competitive (quantitative)

Sensitivity

0.101 ng/ml

Sample type

Serum, plasma, tissue homogenates or other biological fluids.

Detection type

Colourmetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

0.312-20 ng/ml

Assay Time

2h 30m

Principiul testului

Human Diphosphoinositol Polyphosphate Phosphohydrolase 3 beta ELISA Kit (A270844) employs the competitive enzyme immunoassay technique for the quantitative measurement of human Diphosphoinositol Polyphosphate Phosphohydrolase 3 beta in serum, plasma, tissue homogenates or other biological fluids. The 96-well microtiter plate has been pre-coated with Diphosphoinositol Polyphosphate Phosphohydrolase 3 beta antigen. During the incubation, Diphosphoinositol Polyphosphate Phosphohydrolase 3 beta present in the samples or standards competes with the fixed amount of immobilized Diphosphoinositol Polyphosphate Phosphohydrolase 3 beta for binding sites on the Biotinylated Anti-Diphosphoinositol Polyphosphate Phosphohydrolase 3 beta Antibody. The more Diphosphoinositol Polyphosphate Phosphohydrolase 3 beta present in a sample or standard, the less Biotinylated Anti-Diphosphoinositol Polyphosphate Phosphohydrolase 3 beta Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Diphosphoinositol Polyphosphate Phosphohydrolase 3 beta Antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Diphosphoinositol Polyphosphate Phosphohydrolase 3 beta present in each sample or standard. The concentration of Diphosphoinositol Polyphosphate Phosphohydrolase 3 beta can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Storage

Store kit components at +4°C or -20°C as instructed. Do not use past expiration date!

Documentație

• Manual de utilizare (PDF)se deschide pe site-ul producătorului