Human CYP27A1 ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

CYP27A1

Applications

Sandwich ELISA

Reactivity

Human

Recommended dilutions

Validated sample dilutions include HepG2 cell lysate (1:2) and PC-3 cell lysate (undiluted).

Assay type

Sandwich (quantitative)

Sensitivity

0.094 ng/ml

Sample type

Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.

Cross reactivity

Specific for CYP27A1 with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

0.156-10 ng/ml

Assay Time

4 hours

Principiul testului

Human CYP27A1 ELISA Kit (A326516) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human CYP27A1 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for CYP27A1. Standards and samples are added to the wells, where CYP27A1 binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for CYP27A1 is added to the wells. The detection antibody binds the captured CYP27A1 to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of CYP27A1 captured in each well. The concentration of CYP27A1 is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.

Documentație

• Manual de utilizare (PDF)se deschide pe site-ul producătorului