Human Complement C4a ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

48T · 96T

Țintă

Complement C4a

Applications

Sandwich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sensitivity

0.058 ng/ml

Sample type

Serum, plasma or other biological fluids.

Detection type

Colourmetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

0.156-10 ng/ml

Assay Time

4h 30m

Principiul testului

Human Complement C4a ELISA Kit (A82300) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Complement C4a in serum, plasma or other biological fluids. An antibody specific for Complement C4a has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Complement C4a present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Complement C4a Antibody, which binds the captured Complement C4a present in each well. HRP-Avidin conjugate is then added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Complement C4a captured in each well. The concentration of Complement C4a can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Storage

Store kit components at +4°C or -20°C as instructed. Do not use past expiration date!

Documentație

• Manual de utilizare (PDF)se deschide pe site-ul producătorului