Imagine indisponibilăHuman Complement C3 ELISA Kit
Pe scurt
Human Complement C3 ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea Complement C3, cu reactivitate declarată pentru Human. Măsoară în domeniul 7.813-500 ng/ml, are o sensibilitate de 4.688 ng/ml, folosește detecție Colorimetric Detection (OD450) și se poate folosi pe probe de tip Serum, plasma, tissue homogenates, and other biological fluids. Se livrează în mărimea 96T, la prețul de 4.392,30 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Human Complement C3 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Complement C3 in serum, plasma, tissue homogenates, and other biological fluids.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- Complement C3
- Applications
- Sandwich ELISA
- Reactivity
- Human
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 4.688 ng/ml
- Cross reactivity
- Specific for Complement C3 with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 7.813-500 ng/ml
- Assay Time
- 4 hours
- Principiul testului
- Human Complement C3 ELISA Kit (A79910) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Complement C3 in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for Complement C3. Standards and samples are added to the wells, where Complement C3 binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for Complement C3 is added to the wells. The detection antibody binds the captured Complement C3 to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Complement C3 captured in each well. The concentration of Complement C3 is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
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