Human Collagen II ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Collagen II

Applications

Sandwich ELISA

Reactivity

Human

Recommended dilutions

Validated sample dilutions include Cartilage tissue homogenates (undiluted) and U2OS cell lysate (undiluted).

Assay type

Sandwich (quantitative)

Sensitivity

0.375 ng/ml

Sample type

Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.

Cross reactivity

Specific for Collagen II with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

0.625-40 ng/ml

Assay Time

2 hours

Principiul testului

Human Collagen II ELISA Kit (A335705) is a 2 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Collagen II in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. This QuickRun format uses a pre-coated 96-well microtiter plate and optimized ready-to-use reagents to reduce assay time. Standards and samples are added to the wells, where Collagen II binds to the immobilized capture antibody. Assay-specific biotinylated detection antibody and HRP-Streptavidin reagents are then added according to the optimized QuickRun workflow. These reagents form an antibody-antigen-antibody sandwich complex with the captured Collagen II. TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Collagen II captured in each well. The concentration of Collagen II is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.