Human Cleaved Caspase-3 ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Cleaved-caspase 3

Applications

Sandwich ELISA

Reactivity

Human

Recommended dilutions

Serum and plasma samples may affect test results and should be diluted at least 1:2 with Sample Dilution Buffer prior to testing. Validated sample dilutions include Healthy serum (1:2) and K562 cell lysate (1:2).

Assay type

Sandwich (quantitative)

Sensitivity

0.094 ng/ml

Sample type

Serum, plasma, tissue homogenates, and other biological fluids.

Cross reactivity

Specific for Cleaved Caspase-3 with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

0.156-10 ng/ml

Assay Time

4 hours

Principiul testului

Human Cleaved Caspase-3 ELISA Kit (A303114) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Cleaved Caspase-3 in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for Cleaved Caspase-3. Standards and samples are added to the wells, where Cleaved Caspase-3 binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for Cleaved Caspase-3 is added to the wells. The detection antibody binds the captured Cleaved Caspase-3 to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Cleaved Caspase-3 captured in each well. The concentration of Cleaved Caspase-3 is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.

Documentație

• Manual de utilizare (PDF)se deschide pe site-ul producătorului