Imagine indisponibilăAntibodiesA270876
Mouse Chordin ELISA Kit
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Human Chordin ELISA Kit
Pe scurt
Human Chordin ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea Chordin, cu reactivitate declarată pentru Human. Măsoară în domeniul 31.25 - 2000 pg/ml, are o sensibilitate de 18.75 pg/ml și folosește detecție Colorimetric Detection (OD450). Se livrează în mărimea 96T, la prețul de 4.492,13 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Human Chordin ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Chordin in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- Chordin
- Applications
- Sandwich ELISA
- Reactivity
- Human
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 18.75 pg/ml
- Sample type
- Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.
- Cross reactivity
- Specific for Chordin with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 31.25 - 2000 pg/ml
- Assay Time
- 4 hours
- Principiul testului
- Human Chordin ELISA Kit (A326480) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Chordin in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for Chordin. Standards and samples are added to the wells, where Chordin binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for Chordin is added to the wells. The detection antibody binds the captured Chordin to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Chordin captured in each well. The concentration of Chordin is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
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