Human beta Actin ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

beta Actin

Applications

Sandwich ELISA

Reactivity

Human

Recommended dilutions

Validated sample dilutions include SH-SY5Y cell lysate (1:1000-1:2000) and Hela cell lysate (1:1000-1:2000).

Assay type

Sandwich (quantitative)

Sensitivity

0.188 ng/ml

Sample type

Serum, plasma, tissue homogenates, and other biological fluids.

Cross reactivity

Specific for beta Actin with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

0.313-20 ng/ml

Assay Time

4 hours

Principiul testului

Human beta Actin ELISA Kit (A74576) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human beta Actin in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for beta Actin. Standards and samples are added to the wells, where beta Actin binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for beta Actin is added to the wells. The detection antibody binds the captured beta Actin to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of beta Actin captured in each well. The concentration of beta Actin is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.

Documentație

• Manual de utilizare (PDF)se deschide pe site-ul producătorului