Imagine indisponibilăHuman BACE1 ELISA Kit
Pe scurt
Human BACE1 ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea BACE1, cu reactivitate declarată pentru Human. Măsoară în domeniul 0.156-10 ng/ml, are o sensibilitate de 0.094 ng/ml, folosește detecție Colorimetric Detection (OD450) și se poate folosi pe probe de tip Serum, plasma, tissue homogenates, and other biological fluids. Se livrează în mărimea 96T, la prețul de 4.392,30 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Human BACE1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human BACE1 in serum, plasma, tissue homogenates, and other biological fluids.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- BACE1
- Applications
- Sandwich ELISA
- Reactivity
- Human
- Recommended dilutions
- Validated sample dilutions include U-87MG cell lysate (1:2) and SH-5Y5Y cell lysate (1:2).
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 0.094 ng/ml
- Cross reactivity
- Specific for BACE1 with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 0.156-10 ng/ml
- Assay Time
- 4 hours
- Principiul testului
- Human BACE1 ELISA Kit (A75228) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human BACE1 in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for BACE1. Standards and samples are added to the wells, where BACE1 binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for BACE1 is added to the wells. The detection antibody binds the captured BACE1 to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of BACE1 captured in each well. The concentration of BACE1 is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
Documentație
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