Human Anti-21-Hydroxylase Antibody ELISA Kit

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Anti-21-Hydroxylase Antibody

Applications

Sandwich ELISA

Reactivity

Human

Recommended dilutions

Serum and plasma samples may affect test results and should be diluted at least 1:2 with Sample Dilution Buffer prior to testing. Validated sample dilutions include Healthy serum (1:2) and Healthy plasma (1:2).

Assay type

Sandwich (quantitative)

Sensitivity

0.188 ng/ml

Sample type

Serum, plasma, tissue homogenates, and other biological fluids.

Cross reactivity

Demonstrates specificity for anti-21-Hydroxylase antibodies with no reported cross-reactivity to related analogs.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

0.313-20 ng/ml

Assay Time

4 hours

Principiul testului

Human Anti-21-Hydroxylase Antibody ELISA Kit (A73749) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Anti-21-Hydroxylase Antibody in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with assay-specific antigen. Standards and samples are added to the wells, where Anti-21-Hydroxylase Antibody binds to the immobilized antigen. Following incubation, unbound components are removed by washing and a biotinylated antigen detection reagent is added to the wells. This reagent binds the captured Anti-21-Hydroxylase Antibody to form an antigen-antibody-antigen sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Anti-21-Hydroxylase Antibody captured in each well. The concentration of Anti-21-Hydroxylase Antibody is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.