Human Angiopoietin 1 ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Angiopoietin-1

Applications

Sandwich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sensitivity

2.60 pg/ml

Sample type

Cell culture supernatant, serum and plasma.

Cross reactivity

This ELISA kit has shown no significant cross-reactivity.

Detection type

Colorimetric

Platform

Microplate

Product range

31.25 - 2000 pg/ml

Specificitate

This kit can assay both native and recombinant human ANG-1.

Principiul testului

Human Angiopoietin-1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human ANG-1 in the samples. A monoclonal antibody specific for human ANG-1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, and ANG-1 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for ANG-1 is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of ANG-1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Storage

Store at +4°C. Do not use past expiration date!