Human 14-3-3 sigma/SFN ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

48T · 96T

Țintă

14-3-3 sigma/SFN

Applications

Sandwich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sensitivity

0.134 ng/ml

Sample type

Serum, plasma, cerebrospinal fluid or other biological fluids.

Detection type

Colourmetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

0.312-20 ng/ml

Assay Time

4h 30m

Principiul testului

Human 14-3-3 sigma/SFN ELISA Kit (A4929) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human 14-3-3 sigma/SFN in serum, plasma, cerebrospinal fluid or other biological fluids. An antibody specific for 14-3-3 sigma/SFN has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the 14-3-3 sigma/SFN present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-14-3-3 sigma/SFN Antibody, which binds the captured 14-3-3 sigma/SFN present in each well. HRP-Avidin conjugate is then added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of 14-3-3 sigma/SFN captured in each well. The concentration of 14-3-3 sigma/SFN can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Storage

Store kit components at +4°C or -20°C as instructed. Do not use past expiration date!

Documentație

• Manual de utilizare (PDF)se deschide pe site-ul producătorului