Horse POMC ELISA Kit

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

48T · 96T

Țintă

POMC

Applications

Sandwich ELISA

Reactivity

Horse

Assay type

Sandwich (quantitative)

Sensitivity

45 pg/ml

Sample type

Serum, plasma, saliva or other biological fluids.

Detection type

Colourmetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

125-8000 pg/ml

Assay Time

4h 30m

Principiul testului

Horse POMC ELISA Kit (A326186) employs the sandwich enzyme immunoassay technique for the quantitative measurement of horse POMC in serum, plasma, saliva or other biological fluids. An antibody specific for POMC has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the POMC present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-POMC Antibody, which binds the captured POMC present in each well. HRP-Avidin conjugate is then added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of POMC captured in each well. The concentration of POMC can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Storage

Store kit components at +4°C or -20°C as instructed. Do not use past expiration date!

Documentație

• Manual de utilizare (PDF)se deschide pe site-ul producătorului