Imagine indisponibilăELISA Reagents Kit
Pe scurt
ELISA Reagents Kit este un kit ELISA. Folosește detecție Colorimetric și se poate folosi pe probe de tip Serum, plasma, and cell culture supernatants. Se livrează în mărimea 5 x 96T, la prețul de 3.460,60 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
This kit provides all reagents and materials required for optimal assay performance and is supplied in quantities sufficient for five 96-well assays. It is designed to support consistent assay setup, reliable signal development, and reproducible results. The components included are five sets of 96-well strip plates with plate storage materials, one 5 ml vial of 10X Coating Buffer, one 25 ml vial of 200X Wash Buffer, five 10 ml vials of Blocking Buffer, one 12.5 ml vial of 10X Standard Dilution Buffer, one 1 ml vial of 10X Reconstitution Buffer, one 25 ml vial of Secondary Antibody Dilution Buffer, two 25 ml vials of HRP Dilution Buffer, and five 10 ml vials of Stop Reagent.
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Specificații
- Brand
- Antibodies
- Mărime
- 5 x 96T
- Applications
- Sandwhich ELISA
- Conjugate
- Unconjugated
- Detection type
- Colorimetric
- Platform
- Pre-coated Microplate (5 x 96)
- Principiul testului
- ELISA Reagents Kit (A341763) is a sandwich ELISA designed for the sensitive and quantitative measurement of in serum, plasma and cell culture supernatants. The assay utilizes a 96-well microtiter plate pre-coated with an antibody specific for . Standards and samples are added to the wells, allowing present in the samples to bind to the immobilized capture antibody. After washing, a biotinylated detection antibody specific for is added, forming an antibody–antigen–antibody complex. Unbound detection antibody is removed by washing, followed by the addition of streptavidin conjugated to horseradish peroxidase (HRP). Following incubation and washing, TMB substrate is added, resulting in a colorimetric reaction that produces a blue color which turns yellow upon addition of stop solution. The intensity of the color is directly proportional to the concentration of in the sample. The concentration of is determined by measuring the absorbance at 450 nm and comparing the results to the standard curve.
- Storage
- Store at 4°C.
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