Cross-Species TGF beta ELISA Kit

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

TGF beta

Applications

ELISA

Reactivity

Human, Monkey, Mouse, Rat, Rabbit, Porcine, Goat, Bovine, Horse, Donkey, Feline, Canine

Formulation

Antibodies are supplied in Phosphate Buffered Saline with Tris-HCl.

Host

Rabbit

Product form

Recombinant protein is supplied lyophilized; antibodies and buffer are supplied in liquid form.

Assay type

Sandwich (quantitative)

Sensitivity

10.58 pg/ml

Sample type

Serum, plasma and cell culture supernatant.

Detection type

Colorimetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

15.63 pg/ml - 1000 pg/ml

Assay Time

1 hour

Recovery

Cell culture supernatant: 82%; Serum: 107%; Plasma: 101%

Imunogen

The immunogens for the antibodies in this kit are recombinant TGF- beta 1.

Principiul testului

Cross-Species TGF beta ELISA Kit (A334829) employs a one-step sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of human, monkey, mouse, rat, rabbit, porcine, goat, bovine, horse, donkey, feline, canine TGF beta in serum, plasma, and cell culture supernatant. A 96-well microtiter plate is provided pre-coated with an antibody specific for TGF beta. Standards and test samples are added to the wells, allowing TGF beta to bind to the immobilized capture antibody. A prepared antibody mixture is added and incubated to form antibody–antigen complexes. After incubation, unbound material is removed by washing, and TMB substrate is added to develop colour through an HRP-catalysed reaction. The reaction is stopped with acidic stop solution, producing a yellow endpoint signal. The intensity of the colour is proportional to the amount of TGF beta captured in each well. The concentration of TGF beta is determined by measuring absorbance at 450 nm and interpolating from the standard curve.

Storage

Shipped at +4 °C. For short-term storage, keep at +4 °C. Shelf life is 12 months from the date of receipt or reconstitution.