Imagine indisponibilăAntibodiesA103591
Coagulation Factor III Cell Based ELISA Kit
3.826,63 lei3.162,50 leiTVA inclus (21%)fără TVA
Coagulation Factor III (phospho Ser290) Cell Based ELISA Kit
Pe scurt
Coagulation Factor III (phospho Ser290) Cell Based ELISA Kit este un kit ELISA de tip Cell-based (qualitative) pentru măsurarea Tissue Factor, cu reactivitate declarată pentru Human (Ser290). Măsoară în domeniul > 5000 Cells, folosește detecție Colorimetric și se poate folosi pe probe de tip Adherent and suspension cells. Se livrează în mărimea 2 x 96T, la prețul de 4.625,23 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Coagulation Factor III (phospho Ser290) Cell Based ELISA Kit is a Enzyme-Linked Immunosorbent Assay designed for the indirect in vitro quantitative determination of Tissue Factor levels in adherent and suspension cells.
Citește mai multArată mai puțin
Specificații
- Brand
- Antibodies
- Mărime
- 2 x 96T
- Țintă
- Tissue Factor
- Applications
- Cell-Based ELISA
- Reactivity
- Human (Ser290)
- Assay type
- Cell-based (qualitative)
- Sample type
- Adherent and suspension cells.
- Cross reactivity
- No reported cross-reactivity.
- Detection type
- Colorimetric
- Product range
- > 5000 Cells
- Assay Time
- 4 hours 30 minutes
- Principiul testului
- This Coagulation Factor III (phospho Ser290) Cell-Based ELISA Kit allows for the detection of Coagulation Factor III (phospho Ser290) and the effects that certain stimulation conditions have on Coagulation Factor III (phospho Ser290) expression in different cell lines. Qualitative determination of Coagulation Factor III (phospho Ser290) concentration is achieved by an indirect ELISA format. In essence, the Coagulation Factor III (phospho Ser290) is captured by Anti-Coagulation Factor III (phospho Ser290) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this Coagulation Factor III (phospho Ser290) Cell-Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-Coagulation Factor III Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.
Produse similare
- Imagine indisponibilă
- Imagine indisponibilă
- Imagine indisponibilăAntibodiesA101658
Anti-F3 Antibody
1.297,73 – 3.527,15 lei1.072,50 – 2.915,00 leiTVA inclus (21%)fără TVA
- Imagine indisponibilăAntibodiesA121856
Anti-Tissue Factor Antibody [HTF-1] (PE)
2.628,73 lei2.172,50 leiTVA inclus (21%)fără TVA
- Imagine indisponibilăAntibodiesA94983
Anti-Coagulation Factor III (phospho Ser290) Antibody
1.297,73 – 3.527,15 lei1.072,50 – 2.915,00 leiTVA inclus (21%)fără TVA
- Imagine indisponibilăAntibodiesA86828
Anti-Tissue Factor Antibody [HTF-1]
1.331,00 – 2.029,78 lei1.100,00 – 1.677,50 leiTVA inclus (21%)fără TVA
- Imagine indisponibilă
- Imagine indisponibilăAntibodiesA281824
Anti-Tissue Factor Antibody [TF9-10H10]
2.429,08 lei2.007,50 leiTVA inclus (21%)fără TVA
- Imagine indisponibilă
- Imagine indisponibilă
- Imagine indisponibilă
- Imagine indisponibilăAntibodiesA325984
Native Bovine Tissue Factor Protein
4.458,85 – 18.301,25 lei3.685,00 – 15.125,00 leiTVA inclus (21%)fără TVA