Imagine indisponibilăChicken Interferon beta ELISA Kit
Pe scurt
Chicken Interferon beta ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea Interferon beta, cu reactivitate declarată pentru Chicken. Măsoară în domeniul 15.625-1000 pg/ml, are o sensibilitate de 9.375 pg/ml, folosește detecție Colorimetric Detection (OD450) și se poate folosi pe probe de tip Serum, plasma, tissue homogenates, and other biological fluids. Se livrează în mărimea 96T, la prețul de 4.625,23 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Chicken Interferon beta ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of chicken Interferon beta in serum, plasma, tissue homogenates, and other biological fluids.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- Interferon beta
- Applications
- Sandwich ELISA
- Reactivity
- Chicken
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 9.375 pg/ml
- Cross reactivity
- Specific for Interferon beta with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 15.625-1000 pg/ml
- Assay Time
- 4 hours
- Principiul testului
- Chicken Interferon beta ELISA Kit (A74203) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of chicken Interferon beta in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for Interferon beta. Standards and samples are added to the wells, where Interferon beta binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for Interferon beta is added to the wells. The detection antibody binds the captured Interferon beta to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Interferon beta captured in each well. The concentration of Interferon beta is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
Documentație
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