Imagine indisponibilăCEBP beta (phospho Thr235 + Thr188) Cell Based ELISA Kit
Pe scurt
CEBP beta (phospho Thr235 + Thr188) Cell Based ELISA Kit este un kit ELISA de tip Cell-based (qualitative) pentru măsurarea CEBP beta, cu reactivitate declarată pentru Human (Thr235), Mouse (Thr188), Rat (Thr189). Măsoară în domeniul > 5000 Cells, folosește detecție Colorimetric și se poate folosi pe probe de tip Adherent and suspension cells. Se livrează în mărimea 2 x 96T, la prețul de 4.625,23 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
CEBP beta (phospho Thr235 + Thr188) Cell Based ELISA Kit is a Enzyme-Linked Immunosorbent Assay designed for the indirect in vitro quantitative determination of CEBP beta levels in adherent and suspension cells.
Citește mai multArată mai puțin
Specificații
- Brand
- Antibodies
- Mărime
- 2 x 96T
- Țintă
- CEBP beta
- Applications
- Cell-Based ELISA
- Assay type
- Cell-based (qualitative)
- Sample type
- Adherent and suspension cells.
- Cross reactivity
- No reported cross-reactivity.
- Detection type
- Colorimetric
- Product range
- > 5000 Cells
- Assay Time
- 4 hours 30 minutes
- Principiul testului
- This CEBP beta (phospho Thr235 + Thr188) Cell-Based ELISA Kit allows for the detection of CEBP beta (phospho Thr235 + Thr188) and the effects that certain stimulation conditions have on CEBP beta (phospho Thr235 + Thr188) expression in different cell lines. Qualitative determination of CEBP beta (phospho Thr235 + Thr188) concentration is achieved by an indirect ELISA format. In essence, the CEBP beta (phospho Thr235 + Thr188) is captured by Anti-CEBP beta (phospho Thr235 + Thr188) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this CEBP beta (phospho Thr235 + Thr188) Cell-Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-CEBP beta Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.
Produse similare
- Imagine indisponibilă
- Imagine indisponibilă
- Imagine indisponibilăAntibodiesA104108
CEBP beta (phospho Thr235 + Thr188) ELISA Kit
4.758,33 lei3.932,50 leiTVA inclus (21%)fără TVA
- Imagine indisponibilă
- Imagine indisponibilăAntibodiesA95647
Anti-CEBP beta (phospho Thr235 + Thr188) Antibody
1.297,73 – 3.527,15 lei1.072,50 – 2.915,00 leiTVA inclus (21%)fără TVA
- Imagine indisponibilăAntibodiesA95167
Anti-CEBP beta Antibody - Identical to Abcam (ab53138)
1.297,73 – 3.527,15 lei1.072,50 – 2.915,00 leiTVA inclus (21%)fără TVA
- Imagine indisponibilăAntibodiesA85133
Anti-CEBP beta Antibody - Identical to Novus (NBP2-26206)
1.297,73 – 3.527,15 lei1.072,50 – 2.915,00 leiTVA inclus (21%)fără TVA
- Imagine indisponibilă
- Imagine indisponibilăAntibodiesA58497
Anti-CEBP beta Antibody [P47A1AT]
1.963,23 – 5.324,00 lei1.622,50 – 4.400,00 leiTVA inclus (21%)fără TVA
- Imagine indisponibilă
- Imagine indisponibilăAntibodiesA308166
Anti-CEBP Beta (phospho Thr235) Antibody
3.094,58 lei2.557,50 leiTVA inclus (21%)fără TVA
- Imagine indisponibilă