Bovine Total Immunoglobulin ELISA Kit

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Total Immunoglobulin

Applications

Competitive ELISA

Reactivity

Bovine

Recommended dilutions

Validated sample dilutions include Bovine calf serum (4 month) (1:1000) and Bovine calf serum (5 month) (1:1000).

Assay type

Competitive (quantitative)

Sensitivity

0.141 µg/mL

Sample type

Serum, plasma, tissue homogenates, and other biological fluids.

Cross reactivity

Specific for Total Immunoglobulin with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

0.234-15 µg/mL

Assay Time

1 hour

Principiul testului

Bovine Total Immunoglobulin ELISA Kit (A73816) is a 1 hour competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of bovine Total Immunoglobulin in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with Total Immunoglobulin. During the assay, Total Immunoglobulin present in the standards or samples competes with immobilized Total Immunoglobulin for binding to an assay-specific antibody. Following incubation, unbound components are removed by washing and HRP-Streptavidin conjugate is added to the wells. After further incubation and washing, TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the colour is measured at 450 nm using a microplate reader. The concentration of Total Immunoglobulin in the samples is inversely proportional to the OD450 value and is determined by comparison with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.